Photocleavage Test Results

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Summary

• Photocleavage works for IDT internal photocleavable spacers!
• Applications of photo-cleavage can be applied for future use in DNA Origami Containers.
• See also: Photocleavage Protocol

Test 1

• Photocleavage Test 1: Using two strands with internal photocleavable spacers, directly from manufacturer-shipped tubes
• 10% TBE PAGE gel, 15 minute 365 nm UV exposure for "cleaved" strands

Test 2

• Photocleavage Test 2: using gel-purified uncleaved strands from Photocleavage Test 1
  • We did this because there was some background photocleavage in Test 1
  • We also wanted to test a longer exposure to UV light
  • This experiment was successful: we saw nearly complete conversion to the cleaved product.
• 10% TBE PAGE gel, 1 hour 365 nm UV exposure for "cleaved" strands