BME100 f2015:Group17 8amL4: Difference between revisions

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{| style="wikitable" width="700px"
{| style="wikitable" width="700px"
|- valign="top"
|- valign="top"
| [[Image:BME103student.jpg|100px|thumb|Name: Sam Stone]]  
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: Jadyn Ziegler]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: Mahoro Uwiringiyimana]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: Jake Feltz]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: Zachary Ramsey]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: Nathan Natividad]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
|}
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'''Materials'''
'''Materials'''
<!-- Record all of the materials you will need for PCR -->
<!-- Record all of the materials you will need for PCR -->
* Lab coat and disposable gloves
* Item 1
* PCR reaction mix:8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2
* Item 2
, and dNTP’s
* etc.
* DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes
have the same forward primer and reverse primer
*  A strip of empty PCR tubes
* Disposable pipette tips: only use each only once. Never re-use disposable pipette tips or
samples will be cross-contaminated
* Cup for discarded tips
* Micropipettor
* OpenPCR machine: shared by two groups
 




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| G# - || Negative control || none
| G# - || Negative control || none
|-
|-
| G# 1-1 || Patient 1, replicate 1 || 97780
| G# 1-1 || Patient 1, replicate 1 ||  
|-
|-
| G# 1-2 || Patient 1, replicate 2 || 97780
| G# 1-2 || Patient 1, replicate 2 ||  
|-
|-
| G# 1-3 || Patient 1, replicate 3 || 97780
| G# 1-3 || Patient 1, replicate 3 ||  
|-
|-
| G# 2-1 || Patient 2, replicate 1 || 21836
| G# 2-1 || Patient 2, replicate 1 ||  
|-
|-
| G# 2-2 || Patient 2, replicate 2 || 21836
| G# 2-2 || Patient 2, replicate 2 ||  
|-
|-
| G# 2-3 || Patient 2, replicate 3 || 21836
| G# 2-3 || Patient 2, replicate 3 ||  
|}
|}


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<br><br>
<br><br>
==SNP Information & Primer Design==
'''Background: About the Disease SNP'''
<!-- INSTRUCTIONS: This content is from PCR Lab B. Write a summary, at least five sentences long, about the disease SNP in your own words. -->
'''Primer Design and Testing'''
<!-- INSTRUCTIONS: Write a short summary of the results of your primer test. Underneath your summary, include a screen capture of the results web page. You may crop the image so that it only includes the relevant information. -->




Revision as of 08:39, 14 October 2015

BME 100 Fall 2015 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
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OUR TEAM

Name: student
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Name: student
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Name: student
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Name: student
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LAB 4 WRITE-UP

Protocol

Materials

  • Item 1
  • Item 2
  • etc.


PCR Reaction Sample List

Tube Label PCR Reaction Sample Patient ID
G# + Positive control none
G# - Negative control none
G# 1-1 Patient 1, replicate 1
G# 1-2 Patient 1, replicate 2
G# 1-3 Patient 1, replicate 3
G# 2-1 Patient 2, replicate 1
G# 2-2 Patient 2, replicate 2
G# 2-3 Patient 2, replicate 3


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


OpenPCR program






Research and Development

PCR - The Underlying Technology






SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing




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