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- 23:12, 7 May 2024 Altman:WUbites (hist | edit) [2,841 bytes] David Altman (talk | contribs) (Created page with "{{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> =<center>'''WUbites'''</center>= <br> This project is inspired by astrobites, whose goal is to present astrophysical papers in a brief format that is accessible to undergraduate students in the physical sciences. As part of Phys 390W, Science Communication in Physics (SCiP), Willamette Physics Majors sought to translate research articles from a variety of different topics in ph...")
- 08:16, 3 May 2024 BioMicroCenter:Oligo Synthesis (hist | edit) [4,408 bytes] Noelani Kamelamela (talk | contribs) (Created page with "{{BioMicroCenter}} ''' ** All users must be trained before being allowed to use the equipment **'''<BR><BR> The BioMicro Center currently hosts two oligo synthesizers: one Dr Oligo 96 (Biolytic) and one Syntax STX-200 (DNAScript). <br><br> Dr Oligo is optimized for higher concentrations of oligos made through polyamidite synthesis. For ready to use oligos a series of steps must be completed using the other related machines in the Center including the column presser, the...")
- 08:19, 2 May 2024 UA Biophysics:Protocols:Buffer HEPES (hist | edit) [689 bytes] Elizabeth Suesca (talk | contribs) (Created page with "'''Buffer HEPES: 500 mL, pH 7.4 ''' '''Este buffer no puede ser usado para trabajar con Calceina, en ese caso es obligatorio usar un buffer de elución.''' ==Materiales== * HEPES 2,383 g * NaCl 3,1896 g * NaOH 1 M * HCl 1 M ==Procedimiento== # Colocar 400 ml de agua deionizada en la botella con agitador. # Diluir el HEPES y el NaCl # Ajustar pH a 7.4 usando NaOH y/o HCl. # Sacar el agitador y completar los 500 mL con agua deionizada {| class="wikitable" style="...")
- 07:53, 2 May 2024 UA Biophysics:Protocols:Elution Buffer8 mOsM ESP (hist | edit) [1,010 bytes] Elizabeth Suesca (talk | contribs) (Created page with " ==Materiales== * HEPES 9.532 g * NaCl * NaOH 1 M 30 ml * HCl 1 M ==Procedimiento== # Colocar 1.8 litros de agua deionizada en la botella con un agitador. # Diluir el HEPES (concentracion final de 20 mM) y el NaCl (concnetracion final de 170 mM). # Ajustar pH a 7.4 usando NaOH y/o HCl (Generalmente se inicia con pH de 5.4) # Determinar la concentración de HCl y NaOH. Por ejemplo, si entre los dos se agregaron 16 ml a 1M, entonces para un buffer de 2 L la concentrac...")
- 18:52, 1 May 2024 Microfluidic Vasculature for Cell Culture - Lilin Zhao, Melissa Deschamps, Marissa Burgess, Matthew Tiller, Jacob Kellett, Tina Leong, Katelyn Mullen, Daniel Bell, Anna Comperchio, Evelyn Moore (hist | edit) [15,033 bytes] Ejmoore (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} ==Introduction== It is extremely important to create ''in vitro'' models of vasculature in order to study cancer (including metastasis and tumor cell circulation), drug delivery, diseases, and to supply oxygen and remove waste to systems too large to depend on diffusion. Vascular diseases are the leading cause of death worldwide causing around 17 million deaths per year. There are limited treatments, and therefore a dire need to better understa...")
- 08:30, 29 April 2024 UA Biophysics:Protocols:Calcein8 ESP (hist | edit) [1,776 bytes] Elizabeth Suesca (talk | contribs) (Created page with "'''Calceina: 800 mOsM, 50 mM, 50 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * NaCl * 1.5564 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 10 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 1.5564 g de calceina (50 mM o 50 mOsM) # Agregar 200 mM de NaCl (...")
- 12:58, 28 April 2024 Organ/Tumor/Body-on-a-Chip - Organ on a Chip - Michele Caggioni (hist | edit) [56,632 bytes] Michele Caggioni (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} =Introduction= Body-on-a-chip, [https://openwetware.org/wiki/Organ-on-a-chip_-_Dan_Nguyen Organ-on-a-chip.png], and Tumor-on-a-chip all represent a consolidation of microfluidic technologies and biological practices in efforts to model body processes on a higher level of detail and complexity. Through the development of Lab-on-a-chip devices, these microfluidic devices have opened a door that could allow for more human-specific research to occu...")
- 08:43, 26 April 2024 The Paper that Launched Microfluidics - Xi Ning (hist | edit) [16,729 bytes] Xning098 (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} ==Introduction== Microfluidics is the science and technology of systems that process or manipulate small (10 <sup> -18 </sup> to 10 <sup>−18 </sup> litres) amounts of fluids, using channels with dimensions of tens to hundreds of micrometres, as stated by George Whitesides. <sup> https://doi.org/10.1038/nature05058 1 </sup>. Microfluidic devices are microchemical systems such as labs on the chip, organs on the chip and plants on the chip....")
- 16:24, 25 April 2024 Flow and Pattern Asymmetries (hist | edit) [36,602 bytes] Courtneychau (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} == Fundamentals of Mixing == Mixing can be described as a physical process through which two or more components are combined in a way such that a uniform distribution is achieved; it is a fundamental unit operation that is needed for a variety of applications. However, due to differences in macroscale and microscale flow phenomenon, mixing occurs differently, and hence, the design and implementation of mixers also differs greatly between the...")
- 18:42, 16 April 2024 Nanoimprint Lithography (NIL) - Carter Paul (hist | edit) [13,993 bytes] CarterPaul (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} =Motivation= =Introduction to NIL= =Thermal NIL Process=")
- 18:40, 16 April 2024 3D Cell Culture - McLean Taggart, Emma Villares, Maximillian Marek, Scott LeBlanc, Adam Lyons and Jacob Belden (hist | edit) [30,713 bytes] CarterPaul (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} ==Introduction== While most microfluidic devices incorporate a 2D cell culture design, in which a single layer of cells is grown on the bottom of a device, these systems suffer from poor <i>in vivo</i> mimicry, as, in the human body, most cells grow in all directions.<sup>https://doi.org/10.5114/aoms.2016.63743 1</sup> To address this limitation, 3D cell culture devices have been developed - in w...")
- 17:03, 15 April 2024 Multilayer Paper Microfluidics - Madyson Redder (hist | edit) [12,474 bytes] Mredder (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} Overview 3D polymeric or glass microfluidic devices were created to run tests on small amounts of liquid and receive results in a timely manner. However, these devices are costly and time consuming to produce. A solution to this problem was single-layer paper microfluidic devices. The most common known examples of single-layer paper microfluidic devices are pregnancy tests, COVID-19 antigen tests, and glucose test strips. While these devices a...")
- 10:33, 14 April 2024 The paper that launched microfluidics - Xi Ning (hist | edit) [16,816 bytes] Xning098 (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} Microfluidic devices are labs on the chip or microchemical systems or with various applications such as models, reactors, and detectors. They have many benefits that larger scale systems can not achieve. The paper by Harrison et al. is widely recognized as one to establish and popularize microfluidics as a research field, pioneering the phrase lab on a chip and showing its feasibility. <sup>https://doi.org/10.1039/d3lc90076b 2</sup> During...")
- 12:10, 13 April 2024 Cells and Nanoparticles in Flow - Namish Kokkula (hist | edit) [14,824 bytes] Nkokkula (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} == Introduction == == References ==")
- 17:42, 12 April 2024 Free-Boundary Microfluidics - Robert Keane (hist | edit) [25,961 bytes] Rkeane (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} == Introduction: Types of Free-Boundary Microfluidics == == Hydrodynamic == Flow Focusing, Co-flow, Cross flow == Elastohydrodynamics == Electrospray, EHD, Electrospinning == Interfacial Tension == Multi-axial, Oblique, Spinning == Acoustics == Ejection, Acoustophoretic, Vibrating == References == 1.")
- 12:01, 10 April 2024 Microfluidic Vasculature for Cell Culture - Evelyn Moore (hist | edit) [9,278 bytes] Ejmoore (talk | contribs) (Created blank page)
- 07:10, 10 April 2024 Combining Body on a Chip - Recreating the Tumor Microenvironment - Organ on a Chip - Michele Caggioni (hist | edit) [57,786 bytes] Michele Caggioni (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} ==Introduction== Body-on-a-chip, [https://openwetware.org/wiki/Organ-on-a-chip_-_Dan_Nguyen Organ-on-a-chip], and Tumor-on-a-chip all represent a consolidation of microfluidic technologies and biological practices in efforts to model body processes on a higher level of detail and complexity. Through the development of Lab-on-a-chip devices, these microfluidic devices have opened a door that could allow for more human-specific research to occur....")
- 11:22, 9 April 2024 Microfluidics for DNA Sequencing - Khiem Le (hist | edit) [18,925 bytes] Khiemle (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}}")
- 11:20, 2 April 2024 DropBase:Picoinjection chip (hist | edit) [411 bytes] Florian Hollfelder (talk | contribs) (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= '''Description''' Sorter and FF devives. <br> '''Reference''' Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins D.Schnettler et al, bioRxiv, 2023, DOI: 10.1101/2023.02.13.528392 <br> <br> =Downloads= <ol style="list-style-type: lower-roman;"> <li> Chips; dwg <br></li></ol>")
- 11:14, 2 April 2024 DropBase:Sorter and FF devices (hist | edit) [459 bytes] Florian Hollfelder (talk | contribs) (Created page with "=Overview= '''Description''' Sorter and Flow Focussing devices. <br> '''Reference''' Nikolic, N., Anagnostidis, V., Tiwari, A., Chait, R. & Gielen, F. Investigating bacteria-phage interaction dynamics using droplet-based technology. BioRxiv, doi:10.1101/2023.07.14.549014 (2023) <br> <br>")
- 07:39, 2 April 2024 UA Biophysics:Protocols:Elution Buffer ESP (hist | edit) [1,010 bytes] Elizabeth Suesca (talk | contribs) (Created page with " ==Procedimiento== # Colocar 1.6 litros de agua deionizada en la botella con un agitador. # Diluir el HEPES (concentracion final de 20 mM) y el NaCl (concnetracion final de 170 mM). # Ajustar pH a 7.4 usando NaOH y/o HCl. # Determinar la concentración de HCl y NaOH. Por ejemplo, si entre los dos se agregaron 16 ml a 1M, entonces para un buffer de 2 L la concentración es de 8 mM. # Calcular la osmolaridad y completar los 400 mOsm con NaCl. # Sacar el agitador y co...")
- 00:31, 26 March 2024 Orgenizing the data from the LCMS mechine (hist | edit) [410 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "* In order to organize the data for further analysis , we use script in R * Please go over the presentations File:presentation1.ppt, File:presentation2.ppt, File:presentation2.ppt")
- 11:02, 25 March 2024 Designing and testing the qPCR primers (hist | edit) [4,641 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "# For additional information [https://www.dropbox.com/scl/fi/070fhrrw6heimv1xxuxzq/real-time-pcr-handbook-life-technologies-update-flr.pdf?rlkey=eddim7471x9l8uqww1234yg6w&dl=0] ===House keeping genes=== # When checking gene expressed in the BSF it is important to use housekeeping gene as a reference. # Housekeeping genes, also known as reference genes, are a set of genes that are constitutively expressed in cells and are essential for basic cellular functions. # T...")