Yu:TESSX
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| 1. Measure 70mL H2O into a 100mL beaker. | |
| 2. Add 1mL of 1M TRIS (pH 8.0) | 10mM TRIS pH 8.0 |
| 2. Add 200μL of 0.5M EDTA (pH 8.0) | 1mM EDTA pH 8.0 |
| 3. Add 2mL of 5M NaCl | 100mM NaCl |
| 4. Add 10mL of 10% SDS | 1% (w/v) SDS |
| 5. Add 10mL of 20% Triton X-100 | 2% (v/v) Triton X-100 |
| 6. Bring volume to 100mL in a graduated cylinder |