Yu:Super SPO

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Solution recipe for 1L Super SPO sporulation media
1) 2.5g Yeast Extract
2) 15g Potassium Acetate
3) 0.5g Glucose
4) 0.69g Super SPO Amino Acid Powder
5) Bring to volume 1L and sterilize by autoclave

Super SPO Amino Acid Powder
1) 40mg Adenine 40mg Uracil 40mg Tyrosine
2) 20mg Histidine 20mg Leucine 20mg Lysine 20mg Tryptophan 20mg Methionine 20mg Arginine
3) 100mg Phenylalanine
4) 350mg Threonine
5) Combine by mortar & pestle