Yu:SDS PAGE Sample Buffer

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Solution recipe for 6mL 3x SDS-PAGE gel loading buffer
1. Measure 2mL glycerol into a 15mL tube 2mL glycerol
2. Add 4mL 10% SDS 4mL 10% SDS
3. Add .3g dithiothreitol (DTT) .3g DTT
4. Add a small amount of bromophenol blue Pinch of bromophenol blue
5. Aliquot 1mL into eppendorf tubes and store in a -20°C freezer Aliquot and store