Yu:SDS PAGE Sample Buffer

From OpenWetWare
(Redirected from Yu:SDS PAGE SAMPLE BUFFER)
Jump to: navigation, search

Home       Procedures       | Lab Members       | Publications       | Research Interests       | Internal Database       UBLogo.jpg

Solution recipe for 6mL 3x SDS-PAGE gel loading buffer
1. Measure 2mL glycerol into a 15mL tube 2mL glycerol
2. Add 4mL 10% SDS 4mL 10% SDS
3. Add .3g dithiothreitol (DTT) .3g DTT
4. Add a small amount of bromophenol blue Pinch of bromophenol blue
5. Aliquot 1mL into eppendorf tubes and store in a -20°C freezer Aliquot and store