Yu:SDS PAGE Buffer

Solution recipe for 1L 10x SDS-PAGE Buffer. Dilute to 1x for use.

1. Measure 700mL millipore water into a 2L plastic beaker.	700mL H2O
2. Weigh 30.3g Tris base and add it to the mixture	30.3g Tris base
3. Weigh 144.2g glycine and add it to the mixture	144.2g glycine
5. Weigh 10g SDS and add it to the mixture	10g SDS
6. Stir until dissolved
7. Bring volume to 1L with millipore water in a graduated cylinder	Volume to 1L
8. Dilute from 10x to 1x (1:10) for use.