Yu:IPTG

Solution recipe for IPTG(Powder stored at -20⁰C).

Isopropylthio-β-D-galactoside(m.w=238.3)

1. Dissolve a solution of IPTG of 2g of IPTG in 8ml of milipore water H2O

2. Adjust the volume of the solution to 10ml with milipore H2O

3. Sterilize by filtration through a 0.22 micron disposable filter

4. Dispense the solution into 1-ml aliquots and store them at -200C