Yu:FA Lysis buffer

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Solution recipe for 1M Tris( Various pH).
1. 100mM HEPES(40ml of 0.5M stock,pH 7.5).
2. 300mM NaCl(12ml of 5M)
3. 2mM EDTA(0.8ml of 0.5M stock)
4. 2% Trion X-100(4ml of stock bottle)
5. 0.2% DOC (4ml of 10%DOC)
6. Add water to 200 ml
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