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Solution recipe for 500mL Calmodulin-Binding Buffer.
1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker 25mM Tris-HCl
2. Add 15mL of 5M NaCl 150mM NaCl
3. Add 500 μL of 1M Magnesium Acetate 1mM MgOAc
4. Add 500 μL of 1M Imidazole 1mM Imidazole
5. Add 1 mL 1M CaCl2 2mM CaCl2
5. Bring volume to 500mL with millipore H2O in a graduated cylinder Volume to 500mL
6. Complete immediately prior to use with 0.696 μL β-mercaptoethanol per 1 mL CBB 10mM β-mercaptoethanol
7. Keep completed CBB on ice at all times