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Solution recipe for ACTINOMYCIN D.
1. Dissolve 20mg OF ACTINOMYCIN D in 4ml of 100% ethanol.
2. Read the OD440 of the stock solution in ethanol, using 100% ethanol as a blank.
3. The molar extinction coeficient of pure actinomycin D(m.w 1255) in aqueous solution is 21900
4. The absorbance at 440nm of a solution containing 1mg/ml of the drug if 0.182
5. Stock solutions of actinomycin D are stored at -200C in foil-wrapped tubes