YPD and YPAD medium

• Omit agar for liquid culture.
• Agar plates are at 2% rather than the 1.5% usual in bacterial work.
• Dry the plates 2-3 days at RT before storing at 4C for several months.
• Autoclave components for a maximun of 15 minutes at 121C.
• For large volumes, autoclave salts, glucose and agar separately.

YPD (YEPD) medium

A complex medium for routine growth. Available premixed as Difco 0428-17.

• per liter
• Bacto yeast extract (Difco 0127-17) (1%) 10g
• Bacto peptone (Difco 0118-17) (2%) 20g
• Glucose (2%) 20g
• Bacto agar (Difco 0140-01) (2%) 20g

YPAD medium

A complex medium to inhibit reversion of the ade1 and ade2 mutations. As above, with added adenine. Inhibits red coloration in ade strains such as AB1380.

• Bacto yeast extract (Difco 0127-17) (1%) 10g
• Bacto peptone (Difco 0118-17) (2%) 20g
• Glucose (2%) 20g
• Bacto agar (Difco 0140-01) (2%) 20g
• Adenine sulfate (.004%) 40mg

YPG (YPG or YEP-glycerol) medium

A complex medium containing non-fermentable carbon source (glycerol) that does not allow growth of ρ^- or pet mutants.

• per liter
• Bacto yeast extract (Difco 0127-17) (1%) 10g
• Bacto peptone (Difco 0118-17) (2%) 20g
• Glycerol (3% v/v) 30 ml
• Bacto agar (Difco 0140-01) (2%) 20g