Wittrup: Yeast Complex Media

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Liquid, 1000 mL

  • 10g Yeast extract
  • 20g Peptone
  • 20g Dextrose (D-glucose)

Add ddH2O to final volume. Autoclave or filter sterilize. The dextrose may carmelize in the autoclave, which shouldn't hurt the media, but will make it dark. For filter sterilization, it will be very thick, so use a vacu-cap Gelman filter.

Plates, 1000 mL

  • 15g Bacto-agar, in addition to above ingredients

Add ddH2O to final volume. Autoclave.