Wittrup: MES running buffer
From OpenWetWare
Jump to navigationJump to search
Formulation
- 50mM MES
- 50mM Tris Base
- 0.1% SDS
- 1mM EDTA
- pH 7.3
1L of 1x Buffer
- 9.76g MES
- 6.057g Tris Base
- 1g SDS
- 0.292g EDTA
- pH to 7.3
5L of 1x Buffer
- 48.8g MES
- 30.285g Tris Base
- 5g SDS
- 1.461g EDTA
- pH to 7.3