Wittrup: Dishwashing

Glass bottles, glass flasks

• wash in dishwasher on Glassware cycle
• autoclave (30 min sterilization, 30 min dry)
• place on shelves in tissue culture support room (76-286)

Centrifuge bottles, Tunair flasks, plastic beakers

• wash in dishwasher on Plastics cycle
• place centrifuge bottles and Tunair flasks on shelves in tissue culture support room (76-286)
• place plastic beakers in incubator / shaker room (76-284)
• NOTE:

  plastic bottles and flasks are to be autoclaved as needed by the user

  do not wash Tunair cap filters

Spatulas, stir bars

• wash in dishwasher on Glassware cycle
• place in containers on table in incubator / shaker room (76-284)

Pipet tips, microcentrifuge tubes, culture tubes

• autoclave (30 min sterilization, 30 min dry)
• place some on table in main lab (76-291)
• place rest on shelves in dishwasher / autoclave room (76-292)
• recycle empty tip holders in cardboard box
• please empty cardboard box once full (recycling bin located in 76-270)

Pasteur pipettes

• autoclave (30 min sterilization, 30 min dry)
• place on shelves in tissue culture room (76-285)

Biohazard Waste

• autoclave (90 min sterilization)
• allowed to cool, then place each bag into a black plastic bag, secure, and set in hallway
• NOTE:

  just before autoclaving, open up waste bags to allow in steam during cycle

  record tag numbers in biological waste log

Biohazard Sharps

• transport all full sharps bins to the accumulation room
• bring empty bins from the accumulation area to the incubator / shaker room
• NOTE:

  empty bins are stored in the incubator / shaker room (76-284)

  full bins are stored in the incubator / shaker room (76-284)

  the general accumulation area is room 76-268 (key on lab whiteboard)

Gel Buffers

• TAE, MES, MOPS, and transfer buffers are in gel / FPLC room (76-287)
• ensure all buffers are the above refill line throughout the week

By the end of the day on Friday...

• unwashed glassware < 1 load
• biohazard waste < 2 bags