Wittrup: Basic Culture Media

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The following is a recipe for basic cell culture media; some cell lines will require additional components for growth, so it is best to check the literature.

Basic Culture Media

440 mls media (eg: RPMI, AMEM, DMEM)

50 mls FBS (for 10% by volume)

5 mls L-glutamine

5 mls Pen-Strep (optional antibiotic)

L-glutamine, Pen-Strep, and some medias are available from VWR.