WinterVomitingLab:Protocols/Stool suspension

Return to WinterVomitingLab

Overview

Preparation of a 10-20% suspension of virus stock from a stool (or vomit) sample.

Materials

• PBS
• stool or vomit

Procedure

1. To 2 ml screw-cap tubes, add 750 µl of PBS.
2. If the sample is solid or semi-solid, add a pea-sized (~0.1 g) of stool to the PBS. If it is mostly liquid and can be pipetted, add 0.1 ml to the PBS.
3. Vortex thoroughly for ~ 1 min.
4. Centrifuge for 10 min at 10,000 rpm in a microcentrifuge.
5. Collect the stool suspension (supernatant). Note: the pellet can be retained and re-extracted later as it likely will still contain high levels of virus.
6. Stool suspensions can be used immediately or otherwise stored (in aliquots) at -70°C.

References

Relevant papers and books

Contact

• Who has experience with this protocol?

or instead, discuss this protocol.