Wikiomics:BLAST tutorial (exercises)

• blastp with shuffled sequence: note that all hits must be wrong!

>Shuffled_protein
MNALFNGRYVRQNLRFAGFARVDRERPPLTWFQVSAIPLFARGFAGAIDSSRISRSGEAF
VGCVCLAQPCFVGAADGEADLRAGVGSVTVAVNGSLLLFALG

• what is this nucleotide sequence?

>nucleotide_1
ATATAGTTTTTAATTAGGAGGATTTTATCAATGAAAATATCAATACTATACAGTAGCAAGACAGGAAAAA
CCGAAAGAGTTGCCAAGTTAATTGAAGAAGGTGTGAAGAGGTCTGGAAATATTGAAGTAAAAACTATGAA
TTTAGATGCAGTTGATAAAAAGTTCTTACAAGAATCAGAAGGAATTATTTTTGGAACACCAACTTATTAT

• same sequence mutated:

>nucleotide_2
ATATAGTATTTAATTAGGAGGATTTCATCAATGACACTCTCAATACTATACAGTAGCAAG
ACAGGGAAAACAGAAAGAGTTGCCAAGGTAATTGAGGAAGGTGTGAAGAGGTCTCAAAAT
AATGAAGTAAGAACTGTGAATCTAGATGCAGTTCATAATAAGTTCTTCGAAGAATCAGGA
GGCATTCTTTTTGGAACACCAACTTATGAT

• what is it? Any remote similarity?

>protein_1
HIGSDTDGDDDSETEGEWNLKEGGSIDAELSHSRKAKKVESLLRKLHTKDILPEEVSS
GSHLHTRSPMVLFLSLAVPTDKVVMFYKALFTEAELKAPLESLKRFQNNGDARTGKSA
IFMLGGGHFAGAVISHEPVKVKGQLLNEELPYLQKIQSINVLESKTFHRYTTRRKQGG

• play with your favorite gene/protein