WangLab:Ras and Src Treatment Protocol
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Ras and Src Treatment Protocol
- Transfect with adenovirus rasn17 2ug/well (together with adenovirus GFP) in 2% FBS DMEM.
- After 6hrs, replace with 2% FBS DMES.
- Within 48 hr, apply 25ul active src (from upstate) (25*0.055=1.375 ug) together with Dextran #Conjugated to FITC or Texas red (MW 10,000; Molecular Probes, Inc., Eugene, OR) with #Lipofectamine method for 2hr.
- Apply 1% FBS DMES for 1h. fix and harvest the cells.