WangLab:Lysis Buffer
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Lysis Buffer (Non-strigent)
| MQ water | 13.65 ml |
| 2M Tris/Hcl (ph7.5) | 375 ul |
| 5M NaCl | 375 ul |
| Triton X-100 | 150 ul |
| 0.1% SDS | 150 ul |
| 0.5M NaF | 150 ul |
| 100 mM Na3VO4 | 150 ul |
| 100 mM PMSF | 150 ul |
| 10 mg/ml Leupeptin | 15 ul |
| SDS Gel-loading buffer: | 4X | 6X |
| Tris.Cl (PH 6.8) (mM | 200 | 300 |
| Dithiothreltol (DTT, mM) | 400 | 600 |
| SDS (Electrophoresis | 8% | 12% |
| Bromophenol Blue | 0.4% | 0.6% |
| Glycerol | 40% | 60% |
