WangLab:Ligation of Reporter to Adenovirus Ad5
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Ligation of reporter to Adenovirus Ad5
- A+B ligation
| A | B |
| 1. Cut Src reporter with BamHI/EcoRI | 1. pTet cut with EcoRV |
| 2. Purify | 2. Purify and dephosphorylate with CIAP. |
| 3. Klenow +dNTP to fill at 70oC, 15 min | 3. Phenol/chlorut purify |
| 4. Get the insert. | 4. Get the vector. |
