WangLab:Inhibitors

From OpenWetWare
Jump to navigationJump to search

Inhibitors

  1. Jasplakinolide (molecular probes, J-7473): 4 uM 15 min, or 2 M 15 min, 25nM 4h or 50nM 2h give good morphorlogy
  2. ML7: blocking MLCK, 5mM stock, 30 M working concentration.


For HUVECs

  • Cyto D: 0.2 uM, 1 hr
  • Taxol: 0.5 uM, 2 hr
  • Noco: 1 uM, 1 hr


Targeting Molecule Inhibitors Concentration (uM) Duration (min)
Src PP2 10 1 hr
Src PP1 10 1 hr
Src SU6656 10 1 hr
RTK Saurumine 50 1 hr
MAPK PD98… 10 1 hr
FAK AG82… 20 1 hr
FAK NVP-TAE226 1 uM 4 hr?
FLK-1 (VEGFR-2) SU4819 5 uM 1 hr
MicroTubules Colchicine 1 1 hr
MicroTubules Nocodazole 1 1 hr
MicroTubules Paclitaxel (taxol; stablizing) 1 uM 2 hr
Actin filaments Cyto D 1 1 hr
Actin filaments Latrunculin B (sequester G-monomer) 0.5 uM 1 hr
Actin filaments Jasplakinolite (stabilizing) 100 nM 1 hr
PI3K Wortmannin 1 1 hr
Myosin ATPase BDM 10 mM 1 hr
Myosin II Blebbistatin 10 uM 1 hr
ROCK Y27… 10 1 hr
MLCK ML-7; ML-9 10 or 5; 7.5 1 hr
PI3K LY 29… 50 1 hr
apoptosis Pancaspase inhibitor zVAD-fmk 10 uM
de novo protein synthesis cycloheximide 25 ug/ml


Targeting Molecule Stimuli Concentration (uM) Duration (min)
RhoA LPA 200 ng/ml 30 min
Rac PDGF 25 ng/ml 30 min
Cdc42 bradykinin 100 nM 3 min
integrins a5b1, avb3, avb5 Fibronectin 10 ug/ml 1 hr
integrins avb3 Fibronogen, Vitronectin
integrins a1b1, a2b1 collagen
integrins a1b1, a2b1, a3b1 and a6b1 laminin


serum starved for 16 hr. Cells were either treated with LPA (200 ng/ml−1) for 30 min (Sigma, Poole, UK), PDGF (25 ng/ml−1) for 30 min (TCS Biologicals, Botolph Claydon, UK), or bradykinin (100 nM) for 3 min (Sigma) or were plated on fibronectin (BD Biosciences) (10 μg/ml) for 1 hr.