WangLab:HEPES buffer
From OpenWetWare
Jump to navigationJump to search
HEPES
- 4-(2-Hydroxyethyl)-1-piperazineethanesuffonic acid
- C8H18N2O4S
- Molecular weight = 238.30
HEPES buffer
- 20mM HEPES buffer, PH=7.3
- x/(238.30*0.1)*10^3 = 20
- x = 0.4766g
- 100ml H2O
- 1mol/L NaOH
- x’/(40*0.1) = 1.0
- x’ = 4g
- 2g : 50ml H2O, 1g, 25ml H2O
