WangLab:DIC Microscope Setting
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DIC microscope setting:
- Push polarized filter to the right.
- Open the pin hole and field aperture.
- Adjust (turn) the prism (never pull out) under the objective, maintain the slider in for 40-60x obj, pull out for 20x.
- Rotate the Condenser to correct one. 5 for 20x, 4 for 40x, 6 for 60x.