WangLab:Covalently Coupling Beads FN EGF

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Protocol for the coupling beads with Fn or EGF:

  1. Carboxy-functionalized super-paraagnetic beads (1 m, SERA-MAG, #294766050250) were activated with 0.1M sulfo-NHS, 0.1M EDC in 0.1M MES (ph 5.0) for 1 hr at RT.
  2. 2X wash 0.1M MES,
  3. Equilibrate with coupling buffer (0.1M sodium phosphate, ph 8.0)
  4. Coupling at 4oC overnight (
  5. Rinse thoroughly with PBS