WangLab:Coat Glass Bottom Dish with Fibronectin

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Follow this protocol if the transfected cells are to be passed on fibronectin-coated dish one day before imaging.

  • Commercial fibronectin (bFn) has a concentration of 1mg/ml. They need to be diluted 25 times with water to 20ug/ml.
  • Put uncovered dish with fibronectin (200ul/dish) in incubator overnight. Evenly spread the liquid in the well using pipette tip.
  • During passing the cells (right after centrifugation step), pipette out the excess liquid.
  • Pass the cells to the fibronectin covered dishes.

NOTE: The above protocol is for MEF cells. The glass bottom dishes are commercially availabe at CellE&G.