WangLab:Alignment of Microscopy

From OpenWetWare
Jump to navigationJump to search

Alignment of Microscopy

  1. Align the lights, adjust the knob in the light house to position the light in the center.
  2. Align the field.
    • switch eyepiece to O.
    • close down the field aperture.
    • Adjust the knobs to center the field in the center and enlarge it to see whether it can fulfill the field.
  3. Align the phase ring.
    • switch the eyepiece to Boucher, adjust the knob in the eyepiece until see a sharp and clear image.
    • use hand to manually adjust the phase ring until it is centered.
    • Switch the eyepiece to O again, adjust the condenser height.
    • Focus

Taking Images with time lapse video function:

  1. Turn on microscopy, slide the filter to direct the light to a correct camera, turn on controllers, monitor
  2. In the mac computer, go to scion image->special->load macros->time lapse video.txt
  3. Special->start capture->select regions of interest (alt-A for the whole image)->special->save->select numbers of frames based on calculation->select the time intervals->save