WangLab:Alignment of Microscopy

Alignment of Microscopy

1. Align the lights, adjust the knob in the light house to position the light in the center.
2. Align the field.
   • switch eyepiece to O.
   • close down the field aperture.
   • Adjust the knobs to center the field in the center and enlarge it to see whether it can fulfill the field.
3. Align the phase ring.
   • switch the eyepiece to Boucher, adjust the knob in the eyepiece until see a sharp and clear image.
   • use hand to manually adjust the phase ring until it is centered.
   • Switch the eyepiece to O again, adjust the condenser height.
   • Focus

Taking Images with time lapse video function:

1. Turn on microscopy, slide the filter to direct the light to a correct camera, turn on controllers, monitor
2. In the mac computer, go to scion image->special->load macros->time lapse video.txt
3. Special->start capture->select regions of interest (alt-A for the whole image)->special->save->select numbers of frames based on calculation->select the time intervals->save