Waldminghaus:Back Door/Standard Solutions

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The following solutions are good to have available because they are used in many recipes as standard:

Name	Usual size
5M NaCl	200 ml
0.5M EDTA	100 ml

AB Media

Vol	material	Final concentration
200 ml	5xA	1x
400 ml	2xB	1x
10 ml	20% Glucose	0,2%
20 ml	20% Sodium-Acetate	0,4%
50 ml	10% CAA	0,5%
1 ml	10 mg/ml Thiamine	10 µg/ml
2,5 ml	10 mg/ml Uridine	25 µg/ml

Fill up to one liter with sterile H₂O!
You have to choose your carbonesource between Soduim-Acetate, Glucose or Glucose and CAA.