User:Yeem/20.309/Mod 4 lab report

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Figure 1. 1μm beads with z-ratio of 0.5

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Figure 2. Mouse neurons imaged with z-ratio of 3.0

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Figure 3. Red channel of mouse intestine image, corresponding to actin filaments.

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Figure 4. Green channel of mouse intestine image, corresponding to cell nuclei.

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Figure 5. Blue channel of mouse intestine image, corresponding to mucus.

Methods

Jaewon's microscope used a 5-watt 532nm pulse laser to provide excitation. The image dimensions were 256x256 pixels, saved as unsigned 16-bit integers in separate channels. The 3-D images were reconstructed using ImageJ's Volume Viewer plugin.

Discussion

Beads

The beads appeared elongated and slightly deformed, likely an artifact of the field of excitation.

Mouse intestine

A square area in the upper right (x-y plane) was photobleached by accident.