User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/13

From OpenWetWare
Jump to: navigation, search

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


  • Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000)--> SAVE ANTIBODY
  • qPCR Patient material
    • NOTE: F6, F7 & F8 received separate mastermix etc. (7.5μL Mastermix, 0,75μL primer/probe, 6,45 μL RNAse free water) whereas the rest had 14.70 μL of the mastermix etc. (375μL Mastermix, 37,5μL primer/probe, 322,5 μL RNAse free water (50 reactions))
  • Quantify loading and reload?