User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/09/22

From OpenWetWare
Jump to navigationJump to search

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name Main project page
Previous entry      Next entry

Summary

  • Dilution of samples to 20 μg/30 μL (5x) + 10 μL LB per 30 μL sample
' μL μL
Sample ddH2O
Jurkat 25,0 125,0
HEK293 (12/08/2010) 25,4 124,6
HeLa -S (p17 (20.6.2010) 10,7 139,3
S0 (7.5.2010) 97,9 52,1
S1 21,5 128,5
S2 62,0 88,0
S3 22,2 127,8
C0 (7.5.2010) 150,0 0,0
C1 24,6 125,4
C2 14,9 135,1
C3 43,9 106,1
  • Run 1x 8% SDS-PAGE
8% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates
X Jurkat
(30 μL)
HEK293
(30 μL)
HeLa -S
(30 μL)
Marker
(5 μL)
S0
(30 μL)
S1
(30 μL)
S2
(30 μL)
S3
(30 μL)
Marker
(5 μL)
C0
(30 μL)
C1
(30 μL)
C2
(30 μL)
C3
(30 μL)
X
  • Run at 200 V for 40 min.