User:Wilfred J. Poppinga/Notebook/Wilfreds Project/2009/08/12

From OpenWetWare
Jump to navigationJump to search

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name Main project page
Previous entry      Next entry

Jolanda's MBP-ArsR fusion protein

  • Transformation of her ligation failed, as to be expected.

Miscellaneous

  • Restriction MBP-ArsR using non-fast digest
    • 12 μL MBP-Ars
    • 1.5 μL Tango Buffer
    • 0.5 μL XbaI
    • 1 μL PstI
    • total 15 μL
  • 1.5 hour @ 37 °C
  • 80 C

Comments

  • TOPO® TA Cloning® Kit for subcloning .. ask someone



Project name Main project page
Previous entry      Next entry

Entry title

  • TODO Insert content here...

Miscellaneous

  • Something I finished

Comments

  • [leave a comment!]