4/4/13
- The liquid cultures did not grow.
- Prepped two more liquid cultures from the ligation plate, as well as performed another ligation.
> Ligation
|
1 |
2 |
|
Insert DNA (KAH182) |
7.0 |
--- |
|
Vector DNA (KAH201) |
1.7 |
1.7 |
|
2x lgn buf (Roche) |
9.7 |
9.7 |
|
T4 ligase (NEB) |
1.0 |
1.0 |
|
dH2O |
0.6 |
7.6 |
|
|
20 μL |
20 μL |
|
- Follow quick transformation protocol
- Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar
Ligation |
Plate results (lig : neg crtl)04/01/13: Ligation
|
1. KAH201 (S/P)/3403, 50 ng + KAH182 (X/P)/1675, 50 ng |
0
|
2. KAH201 (S/P)/3403, 50 ng |
0
|
- The ligation was unsuccessful.
|