User:Vivian Hernandez
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Vivian Hernandez
College: Massachusetts Institute of Technology
Undergraduate Degree: Biological Engineering, 2009
Contact Info: vivianh at mit.edu
| M13K07 Phage Genome | |||
|---|---|---|---|
| Gene | Notes | Function | Ideas |
| 1 | contains g11 (same stop). Part of g4. | channel inner membrane | change so that p8 can't coat phage and p5 only coats it; see phage survival rate |
| 2 | contains g10 (same stop) | replication + strand | increase p2 production & replication rate |
| 3 | BamH1 site | pilus contacts Tol1A. p3/p6 cap | make pilus contact other protein on bacterial pilus |
| 4 | different in f1 and fd. part in 1, 11. | channel outer (12-14 of them) | modify along with p1 and p11 so more channels produced. |
| 5 | covers single strd dna, replaced by p8. makes protein/dna complex for packaging | more p5 production= more sequestering, less= more single stranded DNA | |
| 6 | accessory to p3. p3/p6 cap | make p6 more surface exposed to allow different binding sites | |
| 7 | stop overlaps with start g9 | blunt end (small) intxn p5 | more copies so that more p5 intxn- more packaging |
| 8 | start overlaps with stop g9 | fully coats phage | produce a little less of this and more p7, p9 so that the surface is made up of more of these, allowing more pore starting points |
| 9 | start g9 overlaps stop g7. Stop g9 with start g8. | blunt end (big) intxn p5 | more copies so more p5 intxn-more packaging |
| 10 | within g2, share stop | regulates # dbl strands, allows + strd accum. | change number of copies to see effect on + strand and dbl strand number |
| 11 | within g1, share stop | channel inner membrane | allow channel to allow strand through only when there is a certain concentration of mature phage to be released |
