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  • RNA Protect (Bacteria) - Qiagen
  • Trizol - Invitrogen
  • Fast Blue - MD (Qbiogene)
  • Chloroform
  • DEPC water
  • 75% ethanol


  • Cell Harvest
  1. Collect 2ml culture and add 4mml of RNA protect (Qiagen) in 15 ml Falcon tube.
  2. Vortex for 5sec, incubate room temperature for 5 min.
  3. Centrifuge for 10min at 7300rpm.
  4. Dab on paper towel (up side down).
  5. Freeze.
  • RNA Isolation
  1. Resuspend the pellet in 1ml Trizol (pipette up and down to resuspend) and transfer to FastB tube.


  1. SDS-PAGE and TRANSFER done as per our lab protocol


1M KH2P04

(No autoclave, no filter, just make it and use it.... in 100ml glass bottle)
13.6gms in 100ml DEPC water