From OpenWetWare

Targetron msa knockout

  • Plasmid prep from msa/control , RN4220 and Dh5
  • Gel shift
    • 4220 didnt work out
    • Dh5 worked out, all colonies picked had the plasmid


  • Used the Dh5 derived retargeted plasmed to transform 4220
  • Plated both msa/control on erm, incubated at 32oC

Cell wall

  • Isolate cell wall from wt/mutant
    • Cells grown overnight in TSB, without antibiotics at 225 rpm and 37oC