User:Tobias Wolfgang Giessen

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Tobias Wolfgang Giessen
  • Tobias Wolfgang Giessen. PhD
  • Leopoldina Postdoctoral Fellow
  • Department of Systems Biology
  • Harvard Medical School
  • 200 Longwood Avenue WAB 536
  • Boston, MA 02115
  • Email me through OpenWetWare

I work in the lab of Pam Silver at Harvard Medical School as a Research Fellow.


  • 2013, PhD, Department of Chemistry, Philipps-University Marburg, Marahiel Group
  • 2010, MS, Department of Chemistry, Philipps-University Marburg, Marahiel Group
  • 2008, Research Internship, Imperial College London, Armstrong Group


  1. Natural Product Biosynthesis
  2. Enzymology
  3. Combinatorial Biosynthesis
  4. Synthetic Biology
  5. Protein Engineering
  6. Carboxysome Assembly
  7. Proteinaceous Compartments: Bacterial Microcompartments, Viral Capsids and Synthetic Metabolons
  8. Self-Assembly in Biology
  9. Using Biology to Create Inorganic Functional Materials


  1. Giessen TW, Kraas FI, and Marahiel MA. A four-enzyme pathway for 3,5-dihydroxy-4-methylanthranilic acid formation and incorporation into the antitumor antibiotic sibiromycin. Biochemistry. 2011 Jun 28;50(25):5680-92. DOI:10.1021/bi2006114 | PubMed ID:21612226 | HubMed [Paper1]
  2. Kraas FI, Giessen TW, and Marahiel MA. Exploring the mechanism of lipid transfer during biosynthesis of the acidic lipopeptide antibiotic CDA. FEBS Lett. 2012 Feb 3;586(3):283-8. DOI:10.1016/j.febslet.2012.01.003 | PubMed ID:22245678 | HubMed [Paper2]
  3. Giessen TW and Marahiel MA. Ribosome-independent biosynthesis of biologically active peptides: Application of synthetic biology to generate structural diversity. FEBS Lett. 2012 Jul 16;586(15):2065-75. DOI:10.1016/j.febslet.2012.01.017 | PubMed ID:22273582 | HubMed [Paper3]
  4. Bosello M, Mielcarek A, Giessen TW, and Marahiel MA. An enzymatic pathway for the biosynthesis of the formylhydroxyornithine required for rhodochelin iron coordination. Biochemistry. 2012 Apr 10;51(14):3059-66. DOI:10.1021/bi201837f | PubMed ID:22439765 | HubMed [Paper4]
  5. Giessen TW, Franke KB, Knappe TA, Kraas FI, Bosello M, Xie X, Linne U, and Marahiel MA. Isolation, structure elucidation, and biosynthesis of an unusual hydroxamic acid ester-containing siderophore from Actinosynnema mirum. J Nat Prod. 2012 May 25;75(5):905-14. DOI:10.1021/np300046k | PubMed ID:22578145 | HubMed [Paper5]
  6. Fl├╝he L, Burghaus O, Wieckowski BM, Giessen TW, Linne U, and Marahiel MA. Two [4Fe-4S] clusters containing radical SAM enzyme SkfB catalyze thioether bond formation during the maturation of the sporulation killing factor. J Am Chem Soc. 2013 Jan 23;135(3):959-62. DOI:10.1021/ja310542g | PubMed ID:23282011 | HubMed [Paper6]
  7. Giessen TW, von Tesmar AM, and Marahiel MA. A tRNA-dependent two-enzyme pathway for the generation of singly and doubly methylated ditryptophan 2,5-diketopiperazines. Biochemistry. 2013 Jun 18;52(24):4274-83. DOI:10.1021/bi4004827 | PubMed ID:23705796 | HubMed [Paper7]
  8. Giessen TW, von Tesmar AM, and Marahiel MA. Insights into the generation of structural diversity in a tRNA-dependent pathway for highly modified bioactive cyclic dipeptides. Chem Biol. 2013 Jun 20;20(6):828-38. DOI:10.1016/j.chembiol.2013.04.017 | PubMed ID:23790493 | HubMed [Paper8]
  9. Giessen TW and Marahiel MA. The tRNA-dependent biosynthesis of modified cyclic dipeptides. Int J Mol Sci. 2014 Aug 21;15(8):14610-31. DOI:10.3390/ijms150814610 | PubMed ID:25196600 | HubMed [Paper9]
  10. Giessen TW, Altegoer F, Nebel AJ, Steinbach RM, Bange G, and Marahiel MA. A synthetic adenylation-domain-based tRNA-aminoacylation catalyst. Angew Chem Int Ed Engl. 2015 Feb 16;54(8):2492-6. DOI:10.1002/anie.201410047 | PubMed ID:25583137 | HubMed [Paper10]
All Medline abstracts: PubMed | HubMed

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