User:Tk/Notebook/MF/2009/03/23

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Genomic DNA Prep

  • all cultures grew
  • plate out cultures on 1161 plates w/o antibiotic
  • prep genomic DNA from 12 ml cultures
  • save 20% glycerol stocks of all cultures
  • potential problem: overgrowth of culture may have killed some of the cultures
  • Use OWW CTAB protocol for genomic DNA scaled up 6x
  • 3.6 ml TE
  • 18 ul of proteinase K
  • 90 ul of 20% SDS (instead of 10%)
  • problem resuspending the pellets