Transformants
- One new transformant on the no-DNA no-BSA plate
- The BSA and DNA transformant from Friday grew cultures in tet medium
- No new transformants from Monday (none expected)
- E. coli transformation showed 62 colonies, down from previously
- probably because I electroporated at 1.5 KV rather than 1.75 KV
New tranformation tests
- transform testing 4x and 40x concentrations with EPB (no glycerol)
- three washes at 45 ml each
- resuspend 50 ul of the 40x concentration in 450 ul for the 4x concentration
- aliquot 3x 50 ul of low and high concentrations
- Add 0, 10 ul, 30 ul of 1 M sorbitol as an adjustment to osmolarity
- Electroporate at 1.5 KV
- outgrowth for 1 hour at 30C
- plate 300 ul
- E. coli transformants done at 1.5 KV and 1.75 KV
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