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OK I have been playing around with magic wand.vi from labview and been using it to track microtubules (which is different apparently than just tubules). Anyways as of right now I can track one tubule through the first 30 images Koch gave me automatically. I don't know what is wrong with the 31st image but the program loses the tubule then. The tube doesn't fade out or bend. So it is strange. I think that the point i choose to select the tubule is a bit off and possibly misses the tubule. Yeah the point i automatically enter misses the tubule. It could be that the best fit line doesn't do a good job of matching the tubule. It could be a coincidence that the point I use just happens to be off the next tubule. I gotta work on this longer, but good news is I can track a tubule through 30 images.