ASHY Multiplex
- prepping to start multiplexing CNHY primers ... so see primer interactions ...
Gradient Summary
- green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
Multiplex Map
- markers placed arbitrarily
- marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
AutoDimer
- color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions
PCR - determine approximate range of each marker
- pool high-quant DNA samples from throughout SD county:
- -- CNHY CPN 02
- -- CNHY LOM 08
- -- CNHY RAJ 01
- -- CNHY RAJ 35
- -- CNHY RAJ 42
- -- CNHY RAJ 50
- -- CNHY TP1 11
- -- CNHY TP3 05
- -- CNHY TP3 19
- -- CNHY TP3 20
- 2µL each DNA STK + 80µL LowTE (to dilute to ~10ng/µL) + 20µL CNHY 062911TKLH ('play' DNA, already 10 ng/µL)
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