Meeting with Rulon - PCR fixes
- try Scun22 primers
- maybe Scun2 just doesn't cross-amplify between Sceloporus undulatus and Sceloporus occidentalis
- check math on dNTPs, ladder (too bright?)
- maybe I didn't make them correctly
- check pH of Tris-Cl and Low TE
- the current working stocks of both are being stored in 50mL conicals instead of glass
- try positive PCR product straight into gel
- is my ladder too concentrated or is my DNA not amplifying
- PCR Rulon's timber rattlers
- Box "C. horridus Primer stocks"
- use #9 (needs rehydration)
- use his Plat Taq, 15mM MgCl2, 10x PCR Buffer, dNTPmix
- Box "C. horridus NH DNA extract 9-17-8"
- extracted DNA, but from different locale
- NanoDrop before using (don't know concentrations)
- try better quality Taq
- may need higher fidelity, or ice wasn't enough
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