User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2012/02/01
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Prepare media for GFP expression next week. Using affinity chromatography, purify the MBP protein with a His-tag, that had previously been attempted to be purified by other members of the class.
Preparation of Media for Expression
Filtering the MBP with His-tag
Groups 2-5 in the previous semester attempted to purify the MBP with His-tag, but did not seem to get much protein. Their purification products were saved and stored at 4°C. the groups saved the fractions from their purification in various "peaks" based on the data they observed from the FPLC. Peaks 3 and 4 will be repurified by our group using affinity chromatography. Over the time they were stored it appeared that bacteria began to grow in some of the collections.
Purifying "Peak 3"
Streaking out E.coli with MBP