User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2015/02/13

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Feb 13 -Glassware

Objectives

  • To sterilize glassware for antibacterial studies
  • To place films from 02/11/2015 into oven to cure
  • To finish characterizing swelling of epoxy films
  • To prepare a new batch of TiO2/epoxy and epoxy alone films that will be cured in a mold appropriate for XRD testing
  • autoclave broth

Measurement of swelling in epoxy film

  • The film made on February 4 was cut into pieces, weighed and measured for thickness, then placed in water until the next lab period
  • Measurements were as follows:
Section Initial thickness (mm) Initial weight (g) Final thickness (mm) Final weight (g) % difference in thickness % increase in weights
1 2.17 2.22551 2.30 2.24013 5.99 0.6569
2 1.78 2.23953 1.78 2.25681 0 0.7716
3 1.65 0.93694 1.66 0.94787 0.61 1.1656
4 2.32 2.30817 2.29 2.32841 -1.29 0.8769

Measurement of swelling in epoxy film with average

  • The film made on February 4 was cut into pieces, weighed and measured for thickness, then placed in water until the next lab period

Swelling test epoxy.PNG

Proposed Protocol for the Antimicrobial Activity of the film

  • Grow the bacteria in broth in ambient temperature for two days/or till the solution becomes cloudy
  • Pipette the bacteria/broth solution onto the film in petri dish
  • Use a gauze patch to collect the solution from the film surface
  • Place the gauze patch in an autoclaved NaCl/KCl solution
  • Pipette solution onto a nutrient agar plate and spread over the surface
  • agar plates should be incubated lid down, at 37C for 24 hours before colonies are counted


LB Broth Media

  • measure 80 mL of water
  • add 2 g of LB Broth
  • autoclave and sterilize in 121C for 60 minutes, and leave for another hour until the pressure drops back to atmosphere pressure