User:Steven Moss/Notebook/AU CHEM-570/2012/03/20

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  • Extract and begin purification of MBP-intein


  • cells were sonicated for 90 seconds with 30 second intervals
  • cells were then centrifuged for 1 hour and 20 minutes at 15,900 rpm.
  • pour supernatant into dialysis tubing for overnight dialysis in 50 mM tris buffer (pH=9.0)
    • 50 mM tris buffer = 60.57 g tris in .5 L of water - 200 mL diluted in 3,800 mL of water, adjusted pH at each step with HCl
  • dialysis done in cold room with constant stirring