Objective
- Extract and begin purification of MBP-intein
Procedure
- cells were sonicated for 90 seconds with 30 second intervals
- cells were then centrifuged for 1 hour and 20 minutes at 15,900 rpm.
- pour supernatant into dialysis tubing for overnight dialysis in 50 mM tris buffer (pH=9.0)
- 50 mM tris buffer = 60.57 g tris in .5 L of water - 200 mL diluted in 3,800 mL of water, adjusted pH at each step with HCl
- dialysis done in cold room with constant stirring
Data
Notes
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