User:Steven J. Koch/Notebook/Kochlab/2009/04/18/Osmolyte unzipping data 2003

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Steve Koch 15:07, 18 April 2009 (EDT): I found a presentation from 2001 showing that I'd tried out sucrose unzipping. I uploaded this to Google Docs:

Preliminary data showed for (among others):

  • Unzipping BsoBI in sucrose (osmolite)
  • Unzipping cross-linked protein-DNA (UV cross-linking of BsoBI and XhoI)
  • Overstretch fluctuations
  • Unzipping BsoBI in high calcium (shows increased unzipping force, and increased non-specific binding)

Steve Koch 16:37, 18 April 2009 (EDT): Also found a whole slew of old presentations. Lots of cool unpublished results in these things. I should start uploading all of them, but I don't want to get too distracted now. In any case, here is a cool one where I show that EcoRI DFS had a cool bimodal distribution:

2003 Presentation summarizing betaine DFS

SJK Betaine DFS Results.png
SJK Betaine Equilibrium Constant.png

Betaine DFS Summary.png