User:Ssutton/Phospholocator/Strains

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Box Name Construct Description Strain Description Method of vector propagation Promoter Terminator
Samantha Box 2: Phospholocator strains SST416GHI-1040 Full-length Cdc28-Phosphoplocator SST1040 (W303a derivative, MATa, can1::HO-CAN1, ho::HO-ADE2, bar1:ura3, ade2, leu2, trp1, his3, CDC28:CDC28-F88A (this is an analog-sensitive Cdc28, inhibitable by 1-NM-PP1), cdc15-2, ura3. We integrated pCTS1-2xmCherry-SV40NLS-ADH1 in at the ura3 locus). Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST429GHI-1040 Full-length S15A-Cdc28-Phospholocator (first serine mutated to alanine) SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST430GHI-1040 Full-length S15D-Cdc28-Phospholocator (first serine mutated to aspartic acid) SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST431GHI-1040 Full-length S33A-Cdc28-Phospholocator (second serine mutated to alanine) SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST432GHI-1040 Full-length S33D-Cdc28-Phospholocator (second serine mutated to aspartic acid) SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST416tGHI-1040 Abridged Cdc28-Phosphoplocator SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST429tGHI-1040 Abridged S15A-Cdc28-Phospholocator (first serine mutated to alanine) SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST430tGHI-1040 Abridged S15D-Cdc28-Phospholocator (first serine mutated to aspartic acid) SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST431tGHI-1040 Abridged S33A-Cdc28-Phospholocator (second serine mutated to alanine) SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST432tGHI-1040 Abridged S33D-Cdc28-Phospholocator (second serine mutated to aspartic acid) SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST445GHI-1040 Full-length Fus3-Phospholocator SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST445tGHI-1040 Abridged Fus3-Phospholocator SST1040 (see above) Integrated at his3 Gal1 Cyc1
Samantha Box 2: Phospholocator strains SST1040 No construct SST1040 (see above)
Samantha Box 2: Phospholocator strains SST1050 No construct SST1050 (same as SST1040, except with wild-type Cdc28)