User:Skosuri/T7.2 tests

This page will track some of my progress in assembling T7.2 sections sent to us by Codon Devices.

Identifying individual sections

T7α

1. T7_1-377s
   
2. T7_347-2051u
   
3. T7_2021-3101s
   
4. T7_3071-3201u
   
5. T7_3171-4431s
   
6. T7_4401-5669s
   
7. T7_5639-6504u
   
8. T7_6474-7540s
   
9. T7_7540-8320u
   

T7.2α

1. T7_1-377s -- 377bp
   
2. T7.2rF_347-2153u -- 1806bp -- construct #4
   
3. T7_2021-3101s -- 1080bp
   
4. T7.2rF_3173-3327u -- 154bp -- construct #2 (well B1)
   
5. T7_3171-4431s -- 1260bp
   
6. T7_4401-5669s -- 1268bp
   
7. T7.2rF_5765-6694u -- 929 -- constuct #10 (well G2)
   
8. T7_6474-7540s -- 1066bp
   
9. T7_7730-8000u -- 300bp -- construct #1 (well A1)
   

Test PCRs

initial amplifications for T7.2 sections

Gel of PCR of all 9 sections of T7.2 for later amplification