User:Sim Huay Ping/Notebook/CBE/08/150/2009/01/13

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

AFM sample preparation

To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated

To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated

Put both microvial into the shaking incubate and leave for 15 mins

Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl

To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge

To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge

Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other

Leave the sample to dry for 15 mins